HEK293 lysate (10ug protein in RIPA buffer) over-expressing human UCN3 with DYKDDDDK tag probed with U2575-03 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with U2575-03 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detection by chemiluminescence.
Supplier Page from United States Biological for Goat Anti-Urocortin 3 Antibody